Entomotoxic Activity of the Extracts from the Fungus, Alternaria tenuissima and Its Major Metabolite, Tenuazonic Acid

The study of fungal antibiotics in their competitive interactions with arthropods may lead to the development novel biorational insecticides. Extracts Alternaria tenuissima MFP253011 obtained using various methods showed a wide range biological activities, including entomotoxic properties. Analysis composition and bioactivity allowed us reveal several known mycotoxins unidentified compounds that be involved activity extracts. Among them, tenuazonic acid (TeA), which was major component A. extracts, found most likely have larvicidal against Galleria mellonella. In intrahaemocoel injection bioassay, TeA toxic G. mellonella Zophobas morio an LT50 6 2 days, respectively, at level 50 µg/larva. Administered orally, inhibited growth larvae caused mortality Acheta domesticus adults (LT50 7 days) concentration 250 µg/g feed. weak contact intestinal two phytophages, Tetranychus urticae Schizaphis graminum, causing 15% 27% 1 mg/mL, respectively. cytotoxic Sf9 cell line (IC50 25 µg/mL). Thus, model insects such as could used for further toxicological characterization TeA.